kam-1325 antibody microarray kits Search Results


kam 1325 antibody microarray chips  (Kinexus Bioinformatics Corporation)
86
Kinexus Bioinformatics Corporation kam 1325 antibody microarray chips
Kam 1325 Antibody Microarray Chips, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kam-1325+antibody+microarray+kits/pm39368789-85-15-19?v=Kinexus+Bioinformatics+Corporation
Average 86 stars, based on 1 article reviews
kam 1325 antibody microarray chips - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
antibody microarray  (Kinexus Bioinformatics Corporation)
86
Kinexus Bioinformatics Corporation antibody microarray
( A ) Experimental workflow used for phosphoproteomic analysis of F508del-CFBE41o- cells treated with control peptide (CP) or PI3Kγ MP (25 μM, 30 minutes). A phospho-specific <t>microarray,</t> containing 875 phosphosite-specific and 451 pan-specific antibodies, was used. Thirty-six proteins showing phosphorylation changes exceeding ±60% compared with the control (expressed as percentage CFC, i.e., percentage fold-change compared with control) were selected for downstream analysis. ( B and C ) Panther Gene Ontology (GO) slim-term enrichment analysis of proteins with altered phosphorylation after PI3Kγ MP treatment. Significantly enriched GO terms (FDR < 0.05) were categorized under ( B ) biological processes and ( C ) cellular components. ( D ) Reactome pathway enrichment analysis of differentially phosphorylated proteins after PI3Kγ MP treatment. Lines represent the top 20 pathways; x axis shows the –log 10 (FDR). Color intensity reflects fold enrichment, and circle size indicates the number of proteins; color intensity (yellow to red) indicates increasing fold enrichment. The full phospho-array protein list served as background reference. ( E ) Proteins with CFC greater than 60% (green) or less than −60% (red). A CFC of 100% corresponds to a 2-fold increase in signal intensity after PI3Kγ MP treatment relative to CP.
Antibody Microarray, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kam-1325+antibody+microarray+kits/pmc13043088-153-35-46?v=Kinexus+Bioinformatics+Corporation
Average 86 stars, based on 1 article reviews
antibody microarray - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
sorcs2 ko glerup  (Taconic Biosciences)
93
Taconic Biosciences sorcs2 ko glerup
Figure 1. Lack of <t>SorCS2</t> hampers BDNF-signaling (A) Identification of downstream targets in the BDNF-signaling cascade and (B) targets of other pathways using a phospho-antibody array in hippocampal neurons (DIV5) from WT and KO mice stimulated with or without BDNF (1 nM, 10 min, n = 2). Hits shown with p < 0.05 and a percentage-change of + -45%. (C and D) Time course of BDNF-induced activation of MAPK (T202/Y204) and RSK (T359/S363) in WT and KO hippocampal neurons (n = 5 of each genotype) as assessed through western blotting and densitometric analysis. Phospho-levels were normalized to beta-actin. Significance was calculated using ordinary two- way ANOVA of main effects, ****p < 0.0001. Data is represented as mean ± SD.
Sorcs2 Ko Glerup, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kam-1325+antibody+microarray+kits/pm40520096-208-193-189?v=Taconic+Biosciences
Average 93 stars, based on 1 article reviews
sorcs2 ko glerup - by Bioz Stars, 2026-07
93/100 stars
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preparation kit illumina rs 122 2001 experimental models  (Illumina Inc)
99
Illumina Inc preparation kit illumina rs 122 2001 experimental models
Figure 1. Lack of <t>SorCS2</t> hampers BDNF-signaling (A) Identification of downstream targets in the BDNF-signaling cascade and (B) targets of other pathways using a phospho-antibody array in hippocampal neurons (DIV5) from WT and KO mice stimulated with or without BDNF (1 nM, 10 min, n = 2). Hits shown with p < 0.05 and a percentage-change of + -45%. (C and D) Time course of BDNF-induced activation of MAPK (T202/Y204) and RSK (T359/S363) in WT and KO hippocampal neurons (n = 5 of each genotype) as assessed through western blotting and densitometric analysis. Phospho-levels were normalized to beta-actin. Significance was calculated using ordinary two- way ANOVA of main effects, ****p < 0.0001. Data is represented as mean ± SD.
Preparation Kit Illumina Rs 122 2001 Experimental Models, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kam-1325+antibody+microarray+kits/pm40520096-208-168-170?v=Illumina+Inc
Average 99 stars, based on 1 article reviews
preparation kit illumina rs 122 2001 experimental models - by Bioz Stars, 2026-07
99/100 stars
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antibody microarrays  (Kinex Pharmaceuticals)
90
Kinex Pharmaceuticals antibody microarrays
Figure 1. Lack of <t>SorCS2</t> hampers BDNF-signaling (A) Identification of downstream targets in the BDNF-signaling cascade and (B) targets of other pathways using a phospho-antibody array in hippocampal neurons (DIV5) from WT and KO mice stimulated with or without BDNF (1 nM, 10 min, n = 2). Hits shown with p < 0.05 and a percentage-change of + -45%. (C and D) Time course of BDNF-induced activation of MAPK (T202/Y204) and RSK (T359/S363) in WT and KO hippocampal neurons (n = 5 of each genotype) as assessed through western blotting and densitometric analysis. Phospho-levels were normalized to beta-actin. Significance was calculated using ordinary two- way ANOVA of main effects, ****p < 0.0001. Data is represented as mean ± SD.
Antibody Microarrays, supplied by Kinex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kam-1325+antibody+microarray+kits/pm37354909-483-15-15?v=Kinex+Pharmaceuticals
Average 90 stars, based on 1 article reviews
antibody microarrays - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Experimental workflow used for phosphoproteomic analysis of F508del-CFBE41o- cells treated with control peptide (CP) or PI3Kγ MP (25 μM, 30 minutes). A phospho-specific microarray, containing 875 phosphosite-specific and 451 pan-specific antibodies, was used. Thirty-six proteins showing phosphorylation changes exceeding ±60% compared with the control (expressed as percentage CFC, i.e., percentage fold-change compared with control) were selected for downstream analysis. ( B and C ) Panther Gene Ontology (GO) slim-term enrichment analysis of proteins with altered phosphorylation after PI3Kγ MP treatment. Significantly enriched GO terms (FDR < 0.05) were categorized under ( B ) biological processes and ( C ) cellular components. ( D ) Reactome pathway enrichment analysis of differentially phosphorylated proteins after PI3Kγ MP treatment. Lines represent the top 20 pathways; x axis shows the –log 10 (FDR). Color intensity reflects fold enrichment, and circle size indicates the number of proteins; color intensity (yellow to red) indicates increasing fold enrichment. The full phospho-array protein list served as background reference. ( E ) Proteins with CFC greater than 60% (green) or less than −60% (red). A CFC of 100% corresponds to a 2-fold increase in signal intensity after PI3Kγ MP treatment relative to CP.

Journal: JCI Insight

Article Title: Targeting PI3K γ anchoring enhances CFTR membrane localization and modulator efficacy via PKD1

doi: 10.1172/jci.insight.198846

Figure Lengend Snippet: ( A ) Experimental workflow used for phosphoproteomic analysis of F508del-CFBE41o- cells treated with control peptide (CP) or PI3Kγ MP (25 μM, 30 minutes). A phospho-specific microarray, containing 875 phosphosite-specific and 451 pan-specific antibodies, was used. Thirty-six proteins showing phosphorylation changes exceeding ±60% compared with the control (expressed as percentage CFC, i.e., percentage fold-change compared with control) were selected for downstream analysis. ( B and C ) Panther Gene Ontology (GO) slim-term enrichment analysis of proteins with altered phosphorylation after PI3Kγ MP treatment. Significantly enriched GO terms (FDR < 0.05) were categorized under ( B ) biological processes and ( C ) cellular components. ( D ) Reactome pathway enrichment analysis of differentially phosphorylated proteins after PI3Kγ MP treatment. Lines represent the top 20 pathways; x axis shows the –log 10 (FDR). Color intensity reflects fold enrichment, and circle size indicates the number of proteins; color intensity (yellow to red) indicates increasing fold enrichment. The full phospho-array protein list served as background reference. ( E ) Proteins with CFC greater than 60% (green) or less than −60% (red). A CFC of 100% corresponds to a 2-fold increase in signal intensity after PI3Kγ MP treatment relative to CP.

Article Snippet: After treatment with 25 μM PI3Kγ MP or an equimolar amount of CP for 30 minutes, F508del-CFBE41o- cells were lysed as described above, and protein samples were frozen at –80°C before being subjected to an antibody microarray (KAM-1325 array) and data analysis, which was performed at Kinexus.

Techniques: Control, Microarray, Phospho-proteomics

Figure 1. Lack of SorCS2 hampers BDNF-signaling (A) Identification of downstream targets in the BDNF-signaling cascade and (B) targets of other pathways using a phospho-antibody array in hippocampal neurons (DIV5) from WT and KO mice stimulated with or without BDNF (1 nM, 10 min, n = 2). Hits shown with p < 0.05 and a percentage-change of + -45%. (C and D) Time course of BDNF-induced activation of MAPK (T202/Y204) and RSK (T359/S363) in WT and KO hippocampal neurons (n = 5 of each genotype) as assessed through western blotting and densitometric analysis. Phospho-levels were normalized to beta-actin. Significance was calculated using ordinary two- way ANOVA of main effects, ****p < 0.0001. Data is represented as mean ± SD.

Journal: iScience

Article Title: A triple serine motif in the intracellular domain of SorCS2 impacts its cellular signaling.

doi: 10.1016/j.isci.2025.112695

Figure Lengend Snippet: Figure 1. Lack of SorCS2 hampers BDNF-signaling (A) Identification of downstream targets in the BDNF-signaling cascade and (B) targets of other pathways using a phospho-antibody array in hippocampal neurons (DIV5) from WT and KO mice stimulated with or without BDNF (1 nM, 10 min, n = 2). Hits shown with p < 0.05 and a percentage-change of + -45%. (C and D) Time course of BDNF-induced activation of MAPK (T202/Y204) and RSK (T359/S363) in WT and KO hippocampal neurons (n = 5 of each genotype) as assessed through western blotting and densitometric analysis. Phospho-levels were normalized to beta-actin. Significance was calculated using ordinary two- way ANOVA of main effects, ****p < 0.0001. Data is represented as mean ± SD.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti human/mouse TrkB R&D Systems AF1494; RRID: AB_2155264 Rabbit anti-mouse MAP2 Millipore AB5622; RRID: AB_91939 Mouse anti-mouse PSD95 Sigma P246; RRID: AB_260911 Rabbit anti-mouse Rab7 Cell Signaling Technology 9367; RRID: AB_1904103 Rabbit anti-mouse Rab11 Cell Signaling Technology 5589; RRID: AB_10693925 Rabbit anti-human/mouse phospho-CREB (S133) Cell Signaling Technology 9198; RRID: AB_2561044 Rabbit anti-human/mouse phospho-MAPK (T202/Y204) Cell Signaling Technology 4370; RRID: AB_2315112 Rabbit anti-human/mouse phospho-RSK (T359/S363) Abcam ab32413; RRID: AB_2181172 Mouse anti-mouse beta-actin Sigma A5441; RRID: AB_476744 HRP-conjugated secondary Rabbit α-mouse DAKO custom made 0260; N/A HRP-conjugated secondary swine α-rabbit DAKO custom made P0217; N/A Rabbit α-mouse SorCS2 DAKO custom made F7378; N/A Donkey anti-goat 568 Life technologies A-11057; RRID: AB_142581 Donkey anti-mouse 488 Life technologies A-21202; RRID: AB_141607 Chemicals, peptides, and recombinant proteins Peptides Pepscan Costum made BDNF Millipore & R&D Systems GF029 & 248-BDB/CF NT3 R&D Systems 267-N3-025 Doxycycline Sigma D9891 DAPT Bio-Techne 2634 Critical commercial assays Kinexus Kinase Microarray Kinexus Bioinformatics KAM-1325 RNeasy Mini Kit Qiagen 74104 TruSeq RNA sample preparation kit Illumina RS-122-2001 Experimental models: Cell lines Human iPSC-derived glutamatergic neurons Bit.bio io1001 Experimental models: Organisms/strains C57BL/6j BomTac wild type Taconic N/A Sorcs2 -/- (Sorcs2 KO) Glerup et al.23 N/A Software and algorithms Zen 2011 Image Processing software Carl Zeiss N/A Imaris software Bitplane N/A Multi Gauge V3.2 software FUJIFILM N/A CiiiDER CiiiDER Software N/A Clustvis Clustvis Software N/A Ingenuity Pathway Analysis QIAGEN Bioinformatics N/A Deposited Data RNA sequencing data Mendeley Mendeley Data: https://doi.org/ 10.17632/wmpwynprhc.1 e1 iScience 28, 112695, June 20, 2025

Techniques: Ab Array, Activation Assay, Western Blot

Figure 2. The SorCS2-tail contains a conserved serine motif phosphorylated upon BDNF-signaling (A) Schematic of SorCS2 full-length (FL) receptor and tailless version. (B) Sequences of wildtype (WT) SorCS2-tail and truncated SorCS2-ICD variants (TC1, TC2, and TC3) and tailless SorCS2. Transmembrane region is marked in red. Numbers indicate amino acid positions. (C) Neuronal branching in response to BDNF (1 nM) in SorCS2 KO hippocampal neurons co-transfected with GFP and truncated variants of SorCS2 receptor (n = 4–14 coverslips per group across two separate experiments with 5–25 neurons analyzed per coverslip, mean branches per neuron showed) and (D) representative images. Scale bars 20–40 μm as shown. Significance of neurite branching experiments was calculated using pairwise t-tests (*p < 0.05, **p < 0.01). Data is represented as mean ± SD.

Journal: iScience

Article Title: A triple serine motif in the intracellular domain of SorCS2 impacts its cellular signaling.

doi: 10.1016/j.isci.2025.112695

Figure Lengend Snippet: Figure 2. The SorCS2-tail contains a conserved serine motif phosphorylated upon BDNF-signaling (A) Schematic of SorCS2 full-length (FL) receptor and tailless version. (B) Sequences of wildtype (WT) SorCS2-tail and truncated SorCS2-ICD variants (TC1, TC2, and TC3) and tailless SorCS2. Transmembrane region is marked in red. Numbers indicate amino acid positions. (C) Neuronal branching in response to BDNF (1 nM) in SorCS2 KO hippocampal neurons co-transfected with GFP and truncated variants of SorCS2 receptor (n = 4–14 coverslips per group across two separate experiments with 5–25 neurons analyzed per coverslip, mean branches per neuron showed) and (D) representative images. Scale bars 20–40 μm as shown. Significance of neurite branching experiments was calculated using pairwise t-tests (*p < 0.05, **p < 0.01). Data is represented as mean ± SD.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti human/mouse TrkB R&D Systems AF1494; RRID: AB_2155264 Rabbit anti-mouse MAP2 Millipore AB5622; RRID: AB_91939 Mouse anti-mouse PSD95 Sigma P246; RRID: AB_260911 Rabbit anti-mouse Rab7 Cell Signaling Technology 9367; RRID: AB_1904103 Rabbit anti-mouse Rab11 Cell Signaling Technology 5589; RRID: AB_10693925 Rabbit anti-human/mouse phospho-CREB (S133) Cell Signaling Technology 9198; RRID: AB_2561044 Rabbit anti-human/mouse phospho-MAPK (T202/Y204) Cell Signaling Technology 4370; RRID: AB_2315112 Rabbit anti-human/mouse phospho-RSK (T359/S363) Abcam ab32413; RRID: AB_2181172 Mouse anti-mouse beta-actin Sigma A5441; RRID: AB_476744 HRP-conjugated secondary Rabbit α-mouse DAKO custom made 0260; N/A HRP-conjugated secondary swine α-rabbit DAKO custom made P0217; N/A Rabbit α-mouse SorCS2 DAKO custom made F7378; N/A Donkey anti-goat 568 Life technologies A-11057; RRID: AB_142581 Donkey anti-mouse 488 Life technologies A-21202; RRID: AB_141607 Chemicals, peptides, and recombinant proteins Peptides Pepscan Costum made BDNF Millipore & R&D Systems GF029 & 248-BDB/CF NT3 R&D Systems 267-N3-025 Doxycycline Sigma D9891 DAPT Bio-Techne 2634 Critical commercial assays Kinexus Kinase Microarray Kinexus Bioinformatics KAM-1325 RNeasy Mini Kit Qiagen 74104 TruSeq RNA sample preparation kit Illumina RS-122-2001 Experimental models: Cell lines Human iPSC-derived glutamatergic neurons Bit.bio io1001 Experimental models: Organisms/strains C57BL/6j BomTac wild type Taconic N/A Sorcs2 -/- (Sorcs2 KO) Glerup et al.23 N/A Software and algorithms Zen 2011 Image Processing software Carl Zeiss N/A Imaris software Bitplane N/A Multi Gauge V3.2 software FUJIFILM N/A CiiiDER CiiiDER Software N/A Clustvis Clustvis Software N/A Ingenuity Pathway Analysis QIAGEN Bioinformatics N/A Deposited Data RNA sequencing data Mendeley Mendeley Data: https://doi.org/ 10.17632/wmpwynprhc.1 e1 iScience 28, 112695, June 20, 2025

Techniques: Transfection

Figure 4. Soluble SorCS1-3 phospho- mimetic tails display neurotrophic activity (A) Schematic of soluble SorCS1 (S1sol-PM), SorCS2 (S2sol-PM) and SorCS3 (S3sol-PM) tails with serine to aspartic acid substitutions as phospho-mimetic motif. (B) Neurite branching of Sorcs2 KO hippocampal neurons transfected with soluble SorCS1-3 mutated tail construct (n = 16–18 coverslips per group across three separate experiments with 5–15 neurons analyzed per coverslip) and (C) representative images. Scale bars 20–40 μm as shown. (D) PSD95 density measured in Sorcs2 KO hip pocampal neurons transfected with soluble SorCS1-3 mutated tail construct (n = 6–8 cover slips per group across two separate experiments with 15–20 neurites analyzed per coverslip) and (E) representative images of PSD95 clusters in neurites. Scale bars 10 μm. ROUT test (Q = 1%) was performed in PDS95 assay to remove potential outliers (three outliers were removed). Significance of neurite branching experiments and PSD95 density was calculated using ordinary one- way ANOVA (*p < 0.05). Data is represented as mean ± SD.

Journal: iScience

Article Title: A triple serine motif in the intracellular domain of SorCS2 impacts its cellular signaling.

doi: 10.1016/j.isci.2025.112695

Figure Lengend Snippet: Figure 4. Soluble SorCS1-3 phospho- mimetic tails display neurotrophic activity (A) Schematic of soluble SorCS1 (S1sol-PM), SorCS2 (S2sol-PM) and SorCS3 (S3sol-PM) tails with serine to aspartic acid substitutions as phospho-mimetic motif. (B) Neurite branching of Sorcs2 KO hippocampal neurons transfected with soluble SorCS1-3 mutated tail construct (n = 16–18 coverslips per group across three separate experiments with 5–15 neurons analyzed per coverslip) and (C) representative images. Scale bars 20–40 μm as shown. (D) PSD95 density measured in Sorcs2 KO hip pocampal neurons transfected with soluble SorCS1-3 mutated tail construct (n = 6–8 cover slips per group across two separate experiments with 15–20 neurites analyzed per coverslip) and (E) representative images of PSD95 clusters in neurites. Scale bars 10 μm. ROUT test (Q = 1%) was performed in PDS95 assay to remove potential outliers (three outliers were removed). Significance of neurite branching experiments and PSD95 density was calculated using ordinary one- way ANOVA (*p < 0.05). Data is represented as mean ± SD.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti human/mouse TrkB R&D Systems AF1494; RRID: AB_2155264 Rabbit anti-mouse MAP2 Millipore AB5622; RRID: AB_91939 Mouse anti-mouse PSD95 Sigma P246; RRID: AB_260911 Rabbit anti-mouse Rab7 Cell Signaling Technology 9367; RRID: AB_1904103 Rabbit anti-mouse Rab11 Cell Signaling Technology 5589; RRID: AB_10693925 Rabbit anti-human/mouse phospho-CREB (S133) Cell Signaling Technology 9198; RRID: AB_2561044 Rabbit anti-human/mouse phospho-MAPK (T202/Y204) Cell Signaling Technology 4370; RRID: AB_2315112 Rabbit anti-human/mouse phospho-RSK (T359/S363) Abcam ab32413; RRID: AB_2181172 Mouse anti-mouse beta-actin Sigma A5441; RRID: AB_476744 HRP-conjugated secondary Rabbit α-mouse DAKO custom made 0260; N/A HRP-conjugated secondary swine α-rabbit DAKO custom made P0217; N/A Rabbit α-mouse SorCS2 DAKO custom made F7378; N/A Donkey anti-goat 568 Life technologies A-11057; RRID: AB_142581 Donkey anti-mouse 488 Life technologies A-21202; RRID: AB_141607 Chemicals, peptides, and recombinant proteins Peptides Pepscan Costum made BDNF Millipore & R&D Systems GF029 & 248-BDB/CF NT3 R&D Systems 267-N3-025 Doxycycline Sigma D9891 DAPT Bio-Techne 2634 Critical commercial assays Kinexus Kinase Microarray Kinexus Bioinformatics KAM-1325 RNeasy Mini Kit Qiagen 74104 TruSeq RNA sample preparation kit Illumina RS-122-2001 Experimental models: Cell lines Human iPSC-derived glutamatergic neurons Bit.bio io1001 Experimental models: Organisms/strains C57BL/6j BomTac wild type Taconic N/A Sorcs2 -/- (Sorcs2 KO) Glerup et al.23 N/A Software and algorithms Zen 2011 Image Processing software Carl Zeiss N/A Imaris software Bitplane N/A Multi Gauge V3.2 software FUJIFILM N/A CiiiDER CiiiDER Software N/A Clustvis Clustvis Software N/A Ingenuity Pathway Analysis QIAGEN Bioinformatics N/A Deposited Data RNA sequencing data Mendeley Mendeley Data: https://doi.org/ 10.17632/wmpwynprhc.1 e1 iScience 28, 112695, June 20, 2025

Techniques: Activity Assay, Transfection, Construct

Figure 7. Hypothetical model describing the mechanistic basis for neurotrophic SorCS2-ICD peptide variants Schematic of the proposed model of SorCS2-ICD in BDNF-signaling in hip pocampal neurons: (1) In wild-type neurons, BDNF binds TrkB-SorCS2 com plex at the post-synaptic site to induce downstream signaling and CREB activation. (2) Mutation of the three serines into alanines in the serine motif of the SorCS2-ICD leads to loss of BDNF induced neurotrophic signaling measured on neurite growth. (3) Serine to aspartate mutations of the serine motif in the SorCS2-ICD is capable of inducing a neurotrophic response even in the absence of BDNF and the SorCS2 extracellular domain. (4) Phospho mimetic peptides of the serine motif (green) attached to a cell-penetrating moiety (yellow, TAT-sequence) elicit biological activity in human neurons by activating CREB and induce neuronal viability independent of BDNF.

Journal: iScience

Article Title: A triple serine motif in the intracellular domain of SorCS2 impacts its cellular signaling.

doi: 10.1016/j.isci.2025.112695

Figure Lengend Snippet: Figure 7. Hypothetical model describing the mechanistic basis for neurotrophic SorCS2-ICD peptide variants Schematic of the proposed model of SorCS2-ICD in BDNF-signaling in hip pocampal neurons: (1) In wild-type neurons, BDNF binds TrkB-SorCS2 com plex at the post-synaptic site to induce downstream signaling and CREB activation. (2) Mutation of the three serines into alanines in the serine motif of the SorCS2-ICD leads to loss of BDNF induced neurotrophic signaling measured on neurite growth. (3) Serine to aspartate mutations of the serine motif in the SorCS2-ICD is capable of inducing a neurotrophic response even in the absence of BDNF and the SorCS2 extracellular domain. (4) Phospho mimetic peptides of the serine motif (green) attached to a cell-penetrating moiety (yellow, TAT-sequence) elicit biological activity in human neurons by activating CREB and induce neuronal viability independent of BDNF.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti human/mouse TrkB R&D Systems AF1494; RRID: AB_2155264 Rabbit anti-mouse MAP2 Millipore AB5622; RRID: AB_91939 Mouse anti-mouse PSD95 Sigma P246; RRID: AB_260911 Rabbit anti-mouse Rab7 Cell Signaling Technology 9367; RRID: AB_1904103 Rabbit anti-mouse Rab11 Cell Signaling Technology 5589; RRID: AB_10693925 Rabbit anti-human/mouse phospho-CREB (S133) Cell Signaling Technology 9198; RRID: AB_2561044 Rabbit anti-human/mouse phospho-MAPK (T202/Y204) Cell Signaling Technology 4370; RRID: AB_2315112 Rabbit anti-human/mouse phospho-RSK (T359/S363) Abcam ab32413; RRID: AB_2181172 Mouse anti-mouse beta-actin Sigma A5441; RRID: AB_476744 HRP-conjugated secondary Rabbit α-mouse DAKO custom made 0260; N/A HRP-conjugated secondary swine α-rabbit DAKO custom made P0217; N/A Rabbit α-mouse SorCS2 DAKO custom made F7378; N/A Donkey anti-goat 568 Life technologies A-11057; RRID: AB_142581 Donkey anti-mouse 488 Life technologies A-21202; RRID: AB_141607 Chemicals, peptides, and recombinant proteins Peptides Pepscan Costum made BDNF Millipore & R&D Systems GF029 & 248-BDB/CF NT3 R&D Systems 267-N3-025 Doxycycline Sigma D9891 DAPT Bio-Techne 2634 Critical commercial assays Kinexus Kinase Microarray Kinexus Bioinformatics KAM-1325 RNeasy Mini Kit Qiagen 74104 TruSeq RNA sample preparation kit Illumina RS-122-2001 Experimental models: Cell lines Human iPSC-derived glutamatergic neurons Bit.bio io1001 Experimental models: Organisms/strains C57BL/6j BomTac wild type Taconic N/A Sorcs2 -/- (Sorcs2 KO) Glerup et al.23 N/A Software and algorithms Zen 2011 Image Processing software Carl Zeiss N/A Imaris software Bitplane N/A Multi Gauge V3.2 software FUJIFILM N/A CiiiDER CiiiDER Software N/A Clustvis Clustvis Software N/A Ingenuity Pathway Analysis QIAGEN Bioinformatics N/A Deposited Data RNA sequencing data Mendeley Mendeley Data: https://doi.org/ 10.17632/wmpwynprhc.1 e1 iScience 28, 112695, June 20, 2025

Techniques: Activation Assay, Mutagenesis, Sequencing, Activity Assay